Coding

Part:BBa_K1354001:Design

Designed by: Lily Lin, Rebecca Poch, Nolana Wong, Sara Wong, Jae Ho Jang   Group: iGEM14_Cooper_Union   (2014-10-03)

VPS75 gene deletion cassette


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 339
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 102
    Illegal BamHI site found at 75
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 339
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 339
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The primers for each step were designed and tested.

Source

TRP1 was contained in pFA6a. Using the protocol primer, the knockout cassette was amplified then used in the transformation of yeast to knock out VPS75. This BioBrick part was taken from the transformed yeast with the VPS75 gene knocked out.

References